| Cortisol

Cortisol is a steroid hormone in the glucocorticoid class of hormones and a stress hormone. When used as medication, it is known as hydrocortisone.It is produced in many animals, mainly by the zona fasciculata of the adrenal cortex in an adrenal gland.In other tissues, it is produced in lower quantities.By a diurnal cycle, cortisol is released and increases in response to stress and a low blood-glucose concentration.It functions to increase blood sugar through gluconeogenesis, suppress the immune system, and aid in the metabolism of calories.It also decreases bone formation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	0.19	0.24	0.31	0.55	0.97	1.38	1.96	2.52

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human Cortisol spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-102	97
Plasma(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Human Cortisol and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	88-105	87-108
	Average (%)	93	98